| 产品编号 | bs-103880P |
| 英文名称 | Recombinant Human PPP1CB Protein, N-His |
| 中文名称 | |
| 别 名 | Serine/threonine-protein phosphatase PP1-beta catalytic subunit; PPP1CD; PP-1B; PPP1CB |
| 克 隆 号 | |
| 理论分子量 | 39.35 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The phosphorylation and de phosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A geoup of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. The protein phosphatases (PP) holoenzyme is a trimertic complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified and are designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX or PP4, is the member of a potentially novel PP family. The PP1 family is composed of subfamily members PP1 alpha, PP1 beta,and PP1 gamma.The PP2A family iscomposed of subfamily members PP2A alpha,and PP2A beta. The PP2B family is composed of subfamily members PP2B-A alpha, PP2B-A beta and PP2B-A gamma. The PP2C family is composed of PP2C alpha, PP2C beta, PP2C gamma. Wip1, a protein isentified in the p53 DNA response pathway, has also been identified as a potential member of the PP2C family. Regulatary subunits include dnuclear inhibitor of PP1(NIPP1), PP2A-AAlphaand–A beta, PP2A-B alpha and B beta,PP2A-C alpha and C beta, PP2A-B56-alpha and–B56 beta, PR48 and PP2B-B1 and–B2. SWISS: P62140 Gene ID: 5500 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |