| 产品编号 | bs-103611P |
| 英文名称 | Recombinant Human AP2A1 Protein, N-His |
| 中文名称 | |
| 别 名 | AP-2 complex subunit alpha-1; Alpha1-adaptin; Adaptor protein complex AP-2 subunit alpha-1; 100 kDa coated vesicle protein A; Clathrin assembly protein complex 2 alpha-A large chain; ADTAA; CLAPA1; AP2A1; Adaptor-related protein complex 2 subunit alpha-1; Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit; Alpha-adaptin A |
| 克 隆 号 | |
| 理论分子量 | 29.38 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression. SWISS: O95782 Gene ID: 161 adaptin α参与披网格蛋白小泡组装的一种蛋白质, 在披网格蛋白小泡组装中与受体的细胞质结构域相互作用, 起衔接作用. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
