| 产品编号 | bs-103536P |
| 英文名称 | Recombinant Human ROBO1 Protein, N-His-SUMO & C-Strep |
| 中文名称 | |
| 别 名 | H-Robo-1; DUTT1; Roundabout homolog 1; Deleted in U twenty twenty; ROBO1 |
| 克 隆 号 | |
| 理论分子量 | 24.54 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior (1). In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively (1). These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway (1). One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals (2,3). Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion (1-3). Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross (1). Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout (2-4). Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray (5,6). SWISS: Q9Y6N7 Gene ID: 6091 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
