| 产品编号 | bs-103512P |
| 英文名称 | Recombinant Human APEX1 Protein, N-His |
| 中文名称 | |
| 别 名 | APX; APE1; APEX nuclease; APEN; Redox factor-1; APE-1; REF1; APEX; APE; Apurinic-apyrimidinic endonuclease 1; HAP1; AP endonuclease 1; APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; REF-1 |
| 克 隆 号 | |
| 理论分子量 | 31.56 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019] SWISS: P27695 Gene ID: 328 转录调节因子（Transcriptin Regulators） APEX(Ref-1)是一种多功能蛋白,除了修复AP位点外,还保持很多转录因子包括AP-1、NF-κB、CREB、ATF、myb、Pax、HIF-1α、HLF、Egr-1、NF-Y、p53和PEBP2等活性还原状态,对维持DNA的稳定和调节细胞因子的表达具有重要作用. APE1是一种具有DNA修复、氧化还原以及调节转录因子活性的多功能蛋白, APE在多种细胞抗氧化损伤过程中起重要的作用。 Ref-1在体内是一种广泛表达的基因,胞质、胞核及胞质 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
