| 产品编号 | bs-103336P |
| 英文名称 | Recombinant Human BBC3 Protein, N-GST & C-His |
| 中文名称 | |
| 别 名 | p53 up-regulated modulator of apoptosis; BBC3; JFY-1; PUMA; Bcl-2-binding component 3; isoforms 1/2 |
| 克 隆 号 | |
| 理论分子量 | 34.49 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] SWISS: Q9BXH1 Gene ID: 27113 PUMA在由p53诱导而经历细胞凋亡的细胞中被p53活化。在线粒体，PUMA 诱导细胞色素C的释放和激活对细胞凋亡的快速诱导。PUMA 与Bcl2、Bcl-XL、类Bcl2-1蛋白相互作用，促使线粒体易位和BAX的多聚化。PUMA在通过依赖细胞色素C/APAF1 途径介导p53诱导的细胞死亡中也发挥作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
