| 产品编号 | bs-102897P |
| 英文名称 | Recombinant Human XRCC5/Ku80 Protein, N-His |
| 中文名称 | |
| 别 名 | Lupus Ku autoantigen protein p86; XRCC5; Thyroid-lupus autoantigen; Ku86; ATP-dependent DNA helicase 2 subunit 2; CTC85; G22P2; DNA repair protein XRCC5; 86 kDa subunit of Ku antigen; TLAA; Nuclear factor IV; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase II 80 kDa subunit; CTCBF; Ku80; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5 |
| 克 隆 号 | |
| 理论分子量 | 41.91 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. SWISS: P13010 Gene ID: 7520 Ku80也是一种DNA修复蛋白，当细胞在受到辐射损伤而发生DNA双链断裂时，Ku80可迅速将其修复，从而提高细胞存活率。 Ku是一种多功能的蛋白，在许多重要的细胞生命过程中起着直接或间接的作用，如DNA双链断裂的修复，免疫球蛋白和T细胞受体V（D）J重排，免疫球蛋白构型转换，DNA复制，DNA转录的调节，同时在细胞周期的G2和M时相中起着特殊的作用。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
