| 产品编号 | bs-102871P |
| 英文名称 | Recombinant Human CYP27B1 Protein, N-His |
| 中文名称 | |
| 别 名 | 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; CYP27B; CYP27B1; Cytochrome P450C1 alpha; Calcidiol 1-monooxygenase; Cytochrome P450VD1-alpha; Cytochrome p450 27B1; CYP1ALPHA; Cytochrome P450 subfamily XXVIIB polypeptide 1; 25-OHD-1 alpha-hydroxylase; VD3 1A hydroxylase; 25-hydroxyvitamin D-1 alpha hydroxylase; mitochondrial |
| 克 隆 号 | |
| 理论分子量 | 30.41 kDa |
| 性 状 | Lyophilized |
| 纯 度 | >90% as determined by SDS-PAGE. |
| 缓 冲 液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| 保存条件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008] SWISS: O15528 Gene ID: 1594 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |