| 产品编号 | bs-24627R |
| 英文名称 | MYCL Rabbit pAb |
| 中文名称 | Myc基因肺癌相关蛋白抗体 |
| 别 名 | l myc protein; l myc 1 proto oncogene; L myc 1 proto-oncogene protein; Myc related gene from lung cancer; MYCL 1; MYCL1; LMYC; BHLHE38; Protein L-Myc-1; oncogene lmyc; v myc avian myelocytomatosis viral oncogene homolog 1 lung carcinoma derived; l-Myc; MYCL1_HUMAN. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human L-Myc: 101-200/364 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis. The MYCL1 gene encodes a protein which is associated with lung cancer in idiopathic pulmonary fibrosis. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an heterodimer with MAX. Subcellular Location: Nucleus. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: P12524 Gene ID: 4610 Database links: Entrez Gene: 4610 Human Entrez Gene: 16918 Mouse Omim: 164850 Human SwissProt: P12524 Human SwissProt: P10166 Mouse Unigene: 437922 Human Unigene: 1055 Mouse Unigene: 479066 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (L-Myc) Polyclonal Antibody, Unconjugated (bs-24627R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (L-Myc) Polyclonal Antibody, Unconjugated (bs-24627R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
