| 产品编号 | bsm-52728R |
| 英文名称 | Rabbit Anti-14-3-3 antibody |
| 中文名称 | 14-3-3蛋白重组兔单抗 |
| 别 名 | 14 3 3 alpha; 14 3 3 protein beta/alpha; 14-3-3 protein beta/alpha; 1433B_HUMAN; Brain protein 14 3 3 beta isoform; GW128; HS 1; KCIP-1; KCIP1; N-terminally processed; Protein 1054; Protein kinase C inhibitor protein 1; YWHAA; YWHAB. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 9C4 |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28/19kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human 14-3-3 protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II, and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It belongs to the 14-3-3 family. Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Subcellular Location: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: The alpha, brain-specific form differs from the beta form in being phosphorylated. Isoform Short contains a N-acetylmethionine at position 1. Similarity: Belongs to the 14-3-3 family. SWISS: P31946 Gene ID: 7529 Database links: Entrez Gene: 7529 Human Omim: 601289 Human SwissProt: P31946 Human Unigene: 643544 Human Unigene: 8653 Rat |
| 产品图片 |
Sample:
Lane 1: human skin tissue lysate
Lane 2: HT-29 cell lysate
Lane 3: NIH/3T3 cell lysate
Primary: Anti-14-3-3 (bsm-52728R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 28/19 kD
Observed band size: 28 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
