| 产品编号 | bsm-52923R |
| 英文名称 | Rabbit Anti-CYP2E1 antibody |
| 中文名称 | 细胞色素P450ⅡE1重组兔单抗 |
| 别 名 | 4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE 1; CPE1; CYP2E; CYP2E1; CYP2E1; CYPIIE1; Cytochrome P450 2E1; cytochrome P450 family 2 subfamily E polypeptide 1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol inducible p450; Ethanol inducible p450; Ethanol-inducible P450; flavoprotein-linked monooxygenase; MGC133529; microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450 J; P450-J; P450C2E; P450C2E; P450J; P450RLM6; xenobiotic monooxygenase. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 脂蛋白 新陈代谢 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 1A26 |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:400-800,IF=1:50-200,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP2E1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and biosynthesis. Cytochrome P450 2E1 is induced by ethanol, diabetes and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, and exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. Function: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Similarity: Belongs to the cytochrome P450 family. SWISS: P05181 Gene ID: 1571 Database links: Entrez Gene: 1571 Human Entrez Gene: 13106 Mouse Omim: 124040 Human SwissProt: P05181 Human SwissProt: Q05421 Mouse SwissProt: P08682 Rabbit Unigene: 12907 Human Unigene: 21758 Mouse Unigene: 1372 Rat |
| 产品图片 |
Sample:
Lane 1: human liver tissue lysates
Primary: Anti-CYP2E1 (bsm-52923R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (bsm-52923R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (bsm-52923R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (bsm-52923R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (bsm-52923R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (bsm-52923R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
