产品编号 | bsm-34014M |
英文名称 | Mouse Anti-Sodium Potassium ATPase antibody |
中文名称 | 钠钾ATP酶蛋白a1单克隆抗体 |
别 名 | ATP1A1; alpha 1 Sodium Potassium ATPase; A1A1; AT1A1; AT1A1_HUMAN; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting subunit alpha 1; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Na,K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1. |
产品类型 | 标签抗体 |
研究领域 | 肿瘤 神经生物学 信号转导 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 2G11 |
交叉反应 | Recombinant protein |
产品应用 | WB=1:2000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 113kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant human Sodium Potassium ATPase: 551-850 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
保存条件 | Store at 2-4 °C. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009]. Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Subunit: nteracts with SIK1. Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Melanosome. Tissue Specificity: Found in most tissues. Post-translational modifications: Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity. Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. SWISS: P05023 Gene ID: 476 Database links: Entrez Gene: 476 Human Entrez Gene: 11928 Mouse Omim: 182310 Human SwissProt: P05023 Human SwissProt: Q8VDN2 Mouse Unigene: 371889 Human Unigene: 280103 Mouse Unigene: 217534 Rat Unigene: 2992 Rat |
产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (bsm-34014M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated(bsm-34014M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |