| 产品编号 | bsm-54219R |
| 英文名称 | Mad2L1 Recombinant Rabbit mAb |
| 中文名称 | Mad2L1重组兔单抗 |
| 别 名 | HSMAD2; Human mitotic feedback control protein Madp2 homolog mRNA complete cds; MAD 2; MAD2 like 1; MAD2 mitotic arrest deficient like 1; MAD2B; MAD2L1; Mitotic arrest deficient 2; Mitotic spindle assembly checkpoint protein MAD2A; REV7; MD2L1_HUMAN. 有丝分裂阻滞缺陷2样蛋白1重组兔单克隆抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 6C6 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Mad2L1 protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008] Function: Component of the spindle-assembly checkpoint thatprevents the onset of anaphase until all chromosomes are properlyaligned at the metaphase plate. Required for the execution of themitotic checkpoint which monitors the process ofkinetochore-spindle attachment and inhibits the activity of theanaphase promoting complex by sequestering CDC20 until allchromosomes are aligned at the metaphase plate. Subunit: Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD during mitosis. Interacts with isoform 1 and isoform 2 of NEK2. Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Post-translational modifications: Phosphorylated on multiple serine residues. The level ofphosphorylation varies during the cell cycle and is highest duringmitosis. Phosphorylation abolishes interaction with MAD1L1 andreduces interaction with CDC20. Phosphorylated by NEK2. Similarity: Belongs to the MAD2 family. Contains 1 HORMA domain. SWISS: Q13257 Gene ID: 4085 Database links: Entrez Gene: 4085 Human Omim: 601467 Human SwissProt: Q13257 Human Unigene: 591697 Human |
| 产品图片 |
Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: SH-SY5Y
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Mad2L1) monoclonal Antibody, Unconjugated (bsm-54219R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
ICC staining Mad2L1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
