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Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid  antibody (bsm-41413M)
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产品编号 bsm-41413M
英文名称 Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid  antibody
中文名称 SARS冠状病毒2核衣壳蛋白单克隆抗体
别    名 SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein.  
Specific References  (4)     |     bsm-41413M has been referenced in 4 publications.
[IF=14.919] Sonnleitner, Sissy Therese. et al. Cumulative SARS-CoV-2 mutations and corresponding changes in immunity in an immunocompromised patient indicate viral evolution within the host. NAT COMMUN. 2022 May;13(1):1-12  ELISA ;  Human.  
[IF=4.188] Sissy Therese Sonnleitner. et al. Maintenance of neutralizing antibodies over ten months in convalescent SARS-CoV-2 afflicted patients. 2021 May 07  Human.  
[IF=4.123] Chunyan Zhang. et al. Foundation and Clinical Evaluation of a New Method for Detecting SARS-CoV-2 Antigen by Fluorescent Microsphere Immunochromatography. Front Cell Infect Mi. 2020; 10: 553837  
[IF=3.388] Sharma Purva. et al. Pathology of COVID 19 associated acute kidney injury. Clin Kidney J. 2021 Jan;:  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 6A8
交叉反应 (predicted: SARS-CoV-2)
产品应用 WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 46kDa
性    状 Liquid
浓    度 ≥1 mg/ml
免 疫 原 Recombinant SARS-CoV-2 Nucleocapsid Protein: full length  
亚    型 IgG1
纯化方法 affinity purified by Protein G
缓 冲 液 0.01M PBS (pH7.4) with 0.02% Proclin300.
保存条件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

SWISS:
P0DTC9

Gene ID:
43740575

产品图片
Sample: Lane 1: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein(Omicron(B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein(WT) at 10ng Primary: Mouse Anti- SARS-CoV-2 N protein (bsm-41413M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kD Observed band size:50 kD
Sample: Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng Primary: Anti- SARS-CoV-2 N protein (bs-41413M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD
Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) (bs-41452P) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) (bs-41492P) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) Primary: Anti-SARS-CoV-2(2019-nCoV)Nucleocapsid(bsm-41413M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 52 kDa
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