产品编号 | bsm-63259R |
英文名称 | Rabbit Anti-eIF4E antibody |
中文名称 | 真核翻译起始因子4E重组兔单抗 |
别 名 | CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E. |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
交叉反应 | Human,Mouse,Rat |
产品应用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200,Flow-Cyt=1:20-1:100,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 25kDa |
检测分子量 | 30 |
性 状 | Liquid |
免 疫 原 | KLH conjugated synthetic peptide derived from human eIF4E |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 |
Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA capping, processing and splicing. EIF4E是蛋白合成的一个主要调控因子,它表达量增加会促进多种肿瘤的生长的增值、分化,因此EIF4E是一种非常重要的抗癌药物靶标。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |