产品编号 | bsm-63199R |
英文名称 | Rabbit Anti-Complement C3 antibody |
中文名称 | 补体C3重组兔单抗 |
别 名 | AHUS5; ARMD9; ASP; Complement C3; Complement factor 3; CPAMD1; HEL S 62p. |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
交叉反应 | Human |
产品应用 | WB=1:1000-1:2000,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 187kDa |
检测分子量 | 115 |
性 状 | Liquid |
免 疫 原 | KLH conjugated synthetic peptide derived from human Complement C3 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 |
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. 补体(complement,C)是由近20种不同血清蛋白组成的多分子系统,约占血清球蛋白总量的10%。补体在血清中的含量相对稳定,不因免疫应答而增加,仅在某些病理情况下才会发生波动。补体系统的基本组成包括9种血清蛋白成分,按发现的先后顺序而分别命名为C1-9。C3是一种β球蛋白,是补体中含量最多、作用最重要的一个组分,是补体两条主要激活途径的中心环节,有重要的生物活性作用,可在肝脏中产生。C3蛋白由α、β2条肽链经二硫键连接而成,本产品能特异性的识别C3的β链。 |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |