| 产品编号 | bsm-63190R |
| 英文名称 | Rabbit Anti-phospho-mTOR(S2448) antibody |
| 中文名称 | 磷酸化雷帕霉素靶蛋白重组兔单抗 |
| 别 名 | FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human,Mouse,Rat,Pig |
| 产品应用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 289kDa |
| 检测分子量 | 289 |
| 性 状 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human mTOR around the phosphorylation site of S2448 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals.MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins.Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2).In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis . mTOR蛋白是一种丝氨酸／苏氨酸蛋白激酶（Ser/Thr），是调节细胞生长和增殖的重要信号转导分子，在细胞的生长、分化、增殖、迁移和存活上扮演了重要的角色。由于mTOR信号通路在细胞周期进程中发挥了重要作用，细胞周期进程调节异常与许多疾病尤其是癌症的发生、发展有关，mTOR信号通路的失调可引起多种肿瘤的发生。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
