| 产品编号 | bsm-63185R |
| 英文名称 | Rabbit Anti-RAF1 antibody |
| 中文名称 | 原癌基因RAF1重组兔单抗 |
| 别 名 | C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 73kDa |
| 检测分子量 | 73 |
| 性 状 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human c Raf |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 |
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. Raf-1（c-Raf）是一种涉及促有丝分裂信号从细胞膜传导到核的蛋白激酶。Raf-1被结合GTP的Ras所征募并与之结合，活化MEK-MAP激酶通路。Raf-1在哺乳动物中通过多个位点的快速高度磷酸化而被活化。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
