| 产品编号 | bsm-52063R-FITC |
| 英文名称 | Cytokeratin 5 Recombinant Rabbit mAb, FITC conjugated |
| 中文名称 | FITC标记的细胞角蛋白5重组兔单抗 |
| 别 名 | Active () Monoclonal Antibody Primary Antibody Unconjugated Rabbit Cytokeratin 5 Unmodified "WB(1:1000-2000)ICC(1:50-200); IHC(1:50-200); " |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human, Mouse, Rat |
| 产品应用 | IF=1:100-500, ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytokeratin 5 Recombinant Rabbit mAb, FITC conjugated |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Cytokeratin 5 Antibody, conjugated (bsm-52063R-FITC) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Cytokeratin 5 Antibody, conjugated (bsm-52063R-FITC) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Cytokeratin 5 Antibody, conjugated (bsm-52063R-FITC) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: Cytokeratin 5 Antibody, conjugated (bsm-52063R-FITC) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
