| 产品编号 | bsm-61822R-Cy3 |
| 英文名称 | DCP1A Recombinant Rabbit mAb, Cy3 conjugated |
| 中文名称 | Cy3标记的脱帽酶1A重组兔单抗 |
| 别 名 | Dcp1a; HSA275986; Nbla00360; SMAD4IP1; SMIF. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human, Mouse (predicted: Rat) |
| 产品应用 | IF=1:50-1:200, Flow-Cyt=1:20-1:100, ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 检测分子量 | 75 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DCP1A Recombinant Rabbit mAb, Cy3 conjugated |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 保存条件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs hDcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both hDcp1a and hDcp2 colocalize in the cytoplasm. In addition, hDcp1a interacts with Smad4 forming a complex with TGF Beta and BMP-4. hDcp1a and Smad4 interact directly through a EVH1/WH1 domain on hDcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of hDcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of hDcp1a eliminates TGF Beta-induced nuclear translocation of hDcp1a. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
