| 产品编号 | D50909 |
| 英文名称 | Heclin |
| 中文名称 | |
| 别 名 | HECT E3 Ubiquitin Ligase抑制剂 |
| 克 隆 号 | |
| CAS | 890605-54-6 |
| 理论分子量 | 283.32 kDa |
| 检测分子量 | |
| 性 状 | Lyophilized |
| 保存条件 | Store in dry, dark place for one year. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
Heclin is a selective HECT E3 ubiquitin ligase inhibitor, inhibits Nedd4, Smurf2 and WWP1 with IC50 6.3, 6.8 and 6.9 μM respectively. It is selective for HECT-mediated over RING-mediated ubiquitination. Heclin does not block E2 binding but causes a conformational change that results in oxidation of the active site Cys. It kills HEK293 cells growing in culture, consistent with an essential role for HECT ligase activity in mammalian cells. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
