| 产品编号 | bsm-34258M |
| 英文名称 | PAPPA Mouse mAb |
| 中文名称 | 妊娠相关血浆蛋白A单克隆抗体 |
| 别 名 | ASBABP2; Aspecific BCL2 ARE binding protein 2; Differentially placenta 1 expressed protein; DIPLA1; IGF dependent IGFBP 4 protease; IGF-dependent IGFBP-4 protease; IGFBP 4ase; IGFBP-4ase; IGFBP4ase; Insulin like growth factor dependent IGF binding protein 4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPP A; PAPP-A; PAPP1_HUMAN; PAPPA; PAPPA1; Pappalysin 1 precursor; Pappalysin-1; Pregnancy Associated Plasma Protein A; Pregnancy associated plasma protein A pappalysin 1; Pregnancy-associated plasma protein A. |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2H5 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | IHC-P=1:100-200,IHC-F=1:100-200,IF=1:100-200,ELISA=1:30-3000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 170 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAPPA |
| 亚 型 | IgG1, k |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Function: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Subunit: Homodimer; disulfide-linked. In pregnancy serum, predominantly found as a disulfide-linked 2:2 heterotetramer with the proform of PRG2. Subcellular Location: Secreted. Tissue Specificity: High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. Post-translational modifications: There appear to be no free sulfhydryl groups. Similarity: Belongs to the peptidase M43B family. Contains 5 Sushi (CCP/SCR) domains. SWISS: Q13219 Gene ID: 5069 Database links: Entrez Gene: 5069 Human Omim: 176385 Human SwissProt: Q13219 Human Unigene: 643599 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAPPA Monoclonal Antibody, Unconjugated(bsm-34258M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, sp-0024) and DAB (C-0010) staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
