产品编号 | 17-0445-01 |
英文名称 | Protein mixture(66-669kDa) (GE) |
中文名称 | 高分子量protein mixture(66-669kDa) (GE) |
别 名 | Amersham High Molecular Weight Calibration Kit for native electrophoresis; Calibration Kit High Molecular Weight For Electrophoresis; Protein Marker; Pre-stained Protein Marker(11-245KDa); Prestained Protein Standards(66-669KDa); Prestained Protein Ladder(66-669KDa); HMW Native Marker(66-669); 蛋白质分子量标准; 蛋白质分子量Marker; 预染蛋白质分子量标准; 彩虹广谱蛋白Marker(66-669KDa); 预染彩虹蛋白marker;分子量蛋白Marker;预染蛋白质分子量marker;彩色预染蛋白分子量; |
保存条件 | The kit should be stored at 2–8°C. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
产品简介 英文名称 HMW Native Marker(66-669) 规格 250ug 用途:用于蛋白质电泳 1. Legal GE and GE monogram are trademarks of General Electric Company. Amersham, Hoefer, Multiphor, PlusOne, PhastGel and PhastSystem are trademarks of GE Healthcare companies.Coomassie is a trademark of Imperial Chemical Industries Ltd© 2006 General Electric Company – All rights reserved. GE Healthcare reserves the right, subject to any regulatory and contractual approval, if required, to make changes in specification and features shown herein, or discontinue the product described at any time without notice or obligation. Contact your GE Healthcare representative for the most current information and a copy of the terms and conditions 2. Handling 2.1. Safety warnings and precautions Warning: For research use only.Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.All chemicals should be considered as potentially hazardous. We therefore recommend that this product is handled only by those persons who have been trained in laboratory techniques and that it is used in accordance with the principles of good laboratory practice. Wear suitable protective clothing, such as laboratory overalls, safety glasses and gloves. Care should be taken to avoid contact with skin or eyes. In the case of contact with skin or eyes, wash immediately with water. See material safety data sheet(s) and/or safety statement(s) for specific advice 2.2. Storage The kit should be stored at 2–8°C. 2.3. Expiry For expiry details see outer packaging. 3. Components Protein mixture 250 μg/vial, 10 vials contains the following proteins:Thyroglobulin (1), porcine thyroid, 76 μg, molecular weight (Mr) 669 000 Ferritin (2), equine spleen, 50 μg, Mr 440 000 Catalase (3), bovine liver, 36 μg, Mr 232 000 Lactate dehydrogenase (4), bovine heart, 48 μg, Mr 140 000. Albumin (5), bovine serum, 40 μg, Mr 66 000. The amount of each protein has been chosen to give bands of equal intensity when stained with Coomassie™ Brilliant Blue following electrophoresis. Intensities may vary when using other staining methods. 4. Other materials required • Electrophoresis reagents appropriate to the application being run. • Detection reagents appropriate to the application being run. • Gel electrophoresis equipment. 5. Critical parameters • Reconstitute the HMW standard vial in appropriate buffer. • Not recommended for use in denaturing systems i.e. containing sodium dodecyl sulphate. 6. Description The High Molecular Weight Calibration Kit is a lyophilized mixture of five highly purified well-characterized proteins for use in molecular weight estimation under non-denaturing conditions. The molecular mass of the protein under investigation is determined by comparing its electrophoretic mobility with that of proteins contained in the kit. Ten vials are supplied, each containing a lyophilized mixture of highly purified protein standards of molecular mass range (Mr) 66 000 to 669 000 7. Protocol 7.1. Preparation of calibration kit Reconstitute the contents of a vial in 100 μl of the electrophoresis sample buffer appropriate to the application being run. When reconstituted in this volume, the protein solution will also contain about 25% sucrose. It is therefore not necessary to add sucrose, glycerol or other density enhancing agents to the sample buffer.For Coomassie Brilliant Blue detectionLoad reconstituted standards without further dilution.For silver stain detectionDilute the reconstituted proteins by at least 50-fold in the electrophoresis sample buffer appropriate to the application being run. 7.2. Gel loading Select the appropriate sample volume from the table:Gel type Sample volume (μl)Vertical mini 5–10Vertical standard 10–20Multiphor™II flatbed 5–20,PhastSystem™ 0.3–4 7.3. Electrophoresis Perform electrophoresis according to the instructions supplied with the gel apparatus being used. 7.4. Detection Stain the gel using the desired method. 7.5. Molecular weight determination The electrophoretic mobility of non-denatured proteins depend on their charge and shape as well as their molecular mass. It is therefore generally not possible to estimate the molecular mass of a non-denaturing protein using the HMW Calibration Kit on a single gel. The molecular mass of a non-denaturing protein can be estimated by running multiple gels of different polyacrylamide concentrations and plotting Rf vs. acrylamide concentration for each standard (Ferguson plots (6,7)). Note: These standards are not recommended for use in denaturing gel electrophoresis in systems containing sodium dodecyl sulphate (SDS, Laemmli gels). Some of the standards consist of multiple subunits and will dissociate under denaturing conditions. Dissociation may not be complete. further complicating interpretation. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |