产品编号 | bsm-34317R |
英文名称 | CREB Recombinant Rabbit mAb |
中文名称 | 环腺苷酸应答元件结合蛋白重组兔单抗 |
别 名 | Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB 1; CREB1; CREB-1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN. |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | 14D4 |
交叉反应 | Human,Mouse (predicted: Rat) |
产品应用 | WB=1:500-1000,IHC-P=1:50-100,IHC-F=1:50-100,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 37 kDa |
检测分子量 | |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CREB |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 | |
产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-CREB (bsm-34317R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 46 kDa
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |