| 产品编号 | bsm-34165M |
| 英文名称 | Placental alkaline phosphatase (PLAP) Mouse mAb |
| 中文名称 | 碱性磷酸酶抗体 |
| 别 名 | Alkaline phosphatase; Alkaline phosphatase placental; Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; ALP; Alp1; ALPP; FLJ61142; Germ-cell alkaline phosphatase; nagao isozyme; OTTHUMP00000164354; PALP; Placental alkaline phosphata |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 5C9 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase |
| 亚 型 | IgG1/kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors. Similarity: Belongs to the alkaline phosphatase family. SWISS: P05187 Gene ID: 250 Database links: Entrez Gene: 250 Human Omim: 171800 Human SwissProt: P05187 Human Unigene: 284255 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Placental alkaline phosphatase (PLAP) monoclonal antibody, unconjugated (bsm-34165M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
