产品编号 | IHC0123 |
英文名称 | PCNA Ready-To-Use IHC Kit |
中文名称 | 增殖细胞核抗原即用型免疫组化试剂盒 |
性 状 | Liquid |
种 属 | Human,Mouse,Rat |
应用范围 | Immunohistochemistry |
适用样品基质 | FFPE tissue |
描述 | IHC0123 is a ready-to-use IHC kit for staining of PCNA. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearanc |
保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. SWISS: P12004 Gene ID: 5111 |
产品图片 |
Immunohistochemical analysis of paraffin embedded mouse testis tissue slide using IHC0123 (PCNA IHC Kit).
Immunohistochemical analysis of paraffin embedded mouse colon tissue slide using IHC0123 (PCNA IHC Kit).
Immunohistochemical analysis of paraffin embedded rat testis tissue slide using IHC0123 (PCNA IHC Kit).
Immunohistochemical analysis of paraffin embedded rat colon tissue slide using IHC0123 (PCNA IHC Kit).
Immunohistochemical analysis of paraffin embedded human small intestine tissue slide using IHC0123 (PCNA IHC Kit).
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |