| 产品编号 | bsm-60777M |
| 英文名称 | phospho-RIPK1(Ser161) Recombinant Mouse mAb |
| 中文名称 | 磷酸化丝氨酸苏氨酸激酶1受体相互作用蛋白单克隆抗体 |
| 别 名 | RIPK1 (phospho-S161); p-RIPK1; phospho-RIPK1; RIPK1 (phospho-Ser161); AIEFL; IMD57; RIP; RIP-1; RIP1; D330015H01Rik; Rinp; RIPK1_HUMAN; RIPK1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); 2.7.11.1; RIPK1_MOUSE; |
| 产品类型 | 磷酸化抗体 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 74 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human RIPK1 around the phosphorylation site of Ser161 |
| 亚 型 | IgG1, k |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains. Function: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Subunit: Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK. Subcellular Location: Cytoplasm. Cell membrane. Tissue Specificity: Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes. Post-translational modifications: Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction. RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylation of Ser-161 by RIPK3 is necessary for the formation of the necroptosis-inducing complex. Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently downregulates TNF-alpha-induced NFkappa-B signaling. Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 death domain. Contains 1 protein kinase domain. SWISS: Q13546 Gene ID: 8737 Database links: Entrez Gene: 8737 Human Entrez Gene: 19766 Mouse Omim: 603453 Human SwissProt: Q13546 Human SwissProt: Q60855 Mouse Unigene: 519842 Human Unigene: 374799 Mouse Unigene: 7572 Rat 受体相互作用蛋白1是一类丝氨酸/苏氨酸蛋白激酶，是一种重要的细胞信号转导分子，他不仅参与了细胞的凋亡，还参与了细胞存活，细胞程序性坏死等多种信号的转导，是一类重要的调节细胞生存或死亡的信号分子，通过激活NF-κB、活化caspase-8、参与活性氧的产生等,在细胞凋亡、细胞存活及细胞程序性坏死等信号传导中起关键作用,其功能受泛素化、锌指蛋白及热休克蛋白等的调节。 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
