产品编号 | bs-42142R |
英文名称 | Rabbit Anti-DSN1 antibody |
中文名称 | 着丝粒相关蛋白DSN1抗体 |
别 名 | C20orf172; dJ469A13.2; DSN1; DSN1, MIND kinetochore complex component, homolog; DSN1, MIND kinetochore complex component, homolog (S. cerevisiae); DSN1_HUMAN; FLJ13346; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore-associated protein DSN1 homolog; MGC32987; MIS13. |
研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human (predicted: Rabbit,Sheep,Cow,Dog,Horse) |
产品应用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 40kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | Recombinant human DSN1 protein |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a kinetochore protein that functions aspart of the minichromosome instability-12 centromere complex. Theencoded protein is required for proper kinetochore assembly andprogression through the cell cycle. Alternative splicing results inmultiple transcript variants. [provided by RefSeq, Feb 2009]. SWISS: Q9H410 Gene ID: 79980 |
产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1 ) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DSN1 ) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |