产品编号 | bsm-52727R |
英文名称 | Rabbit Anti-IL-1 beta antibody |
中文名称 | 白介素1β/IL-1β重组兔单抗 |
别 名 | IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta; IL1F2; IL-1 beta; Catabolin; IL-1; IL1F2; IL1beta; IL1-BETA; IL-1beta; IL-1 Beta; IL-1β; IL1β; IL-1B; IL1B; IL-1B; IL-1 ß; IL-1-ß; IL-1 ß; |
研究领域 | 细胞生物 免疫学 糖蛋白 |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | 1T3 |
交叉反应 | Human |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 31kDa |
细胞定位 | 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human IL-1 beta |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4)with 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. SWISS: P01584 Gene ID: 3553 |
产品图片 |
Sample:
Lane 1: Human THP-1 cell lysates
Lane 2: Human THP-1 treated with 80nM TPA for 24h, then treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours
Primary: Anti-IL-1 beta (bsm-52727R) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 35 kDa
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |