| 产品编号 | bsm-43075M |
| 英文名称 | Mouse Anti-Human CGA antibody |
| 中文名称 | 人绒毛膜促性腺激素α亚基单克隆抗体 |
| 别 名 | GLHA_HUMAN; Glycoprotein hormones alpha chain; Anterior pituitary glycoprotein hormones common subunit alpha; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; Choriogonadotropin alpha chain; Chorionic gonadotrophin subunit alpha(CG-alpha); Follicle-stimulating hormone alpha chain(FSH-alpha); Follitropin alpha chain; Luteinizing hormone alpha chain(LSH-alpha); Lutropin alpha chain; Thyroid-stimulating hormone alpha chain(TSH-alpha); Thyrotropin alpha chain; glycoprotein hormones, alpha polypeptide; HCG alpha; |
| 研究领域 | 肿瘤 免疫学 生长因子和激素 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 9H5 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13 kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 免 疫 原 | Recombinant Human FSH Protein: 25-116/116 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M PBS (pH7.4). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] SWISS: P01215 Gene ID: 1081 |
| 产品图片 |
Sample:
Lane 1: Recombinant human CGA protein, His (HEK293)( bs-43075P)
Primary: Anti-Human CGA (bsm-43075M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 23 kDa
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Human CGA) Monoclonal Antibody, Unconjugated (bsm-43075M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
