| 产品编号 | bsm-54427R |
| 英文名称 | Retinoid X Receptor alpha Recombinant Rabbit mAb |
| 中文名称 | 核受体RXRα重组兔单抗 |
| 别 名 | NR2B1; RXR-alpha; RXRalpha; 9530071D11Rik; RXRalpha1; RXRA_HUMAN; RXRA; Nuclear receptor subfamily 2 group B member 1; Retinoid X receptor alpha; RXRA_MOUSE; RXRA_RAT; |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 细胞膜受体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 5F5 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | 55 |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Retinoid X Receptor alpha protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]. SWISS: P19793 Gene ID: 6256 |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with Retinoid X Receptor alpha monoclonal antibody, unconjugated (bsm-54427R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Retinoid X Receptor alpha antibody (bsm-54427R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
