产品编号 | bsm-52436R |
英文名称 | Rabbit Anti-Calreticulin antibody |
中文名称 | 钙网蛋白重组兔单抗 |
别 名 | Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA. |
研究领域 | 信号转导 细胞类型标志物 |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | 3D1 |
交叉反应 | Human,Mouse,Rat |
产品应用 | WB=1:200-500,IHC-P=1:100-500,ICC=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 44kDa |
细胞定位 | 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Calreticulin |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] SWISS: P27797 Gene ID: 811 |
产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Mouse Testis tissue lysates
Lane 5: Mouse Heart tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Muscle tissue lysates
Lane 8: Rat Testis tissue lysates
Lane 9: Rat Heart tissue lysates
Lane 10: Human SH-SY5Y cell lysates
Lane 11: Human HeLa cell lysates
Lane 12: Human K562 cell lysates
Lane 13: Human HL-60 cell lysates
Lane 14: Human A431 cell lysates
Primary: Anti-Calreticulin (bsm-52436R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 58 kDa
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-52436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-52436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-52436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-52436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (bsm-52436R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |