| 产品编号 | bsm-60744R |
| 英文名称 | Rabbit Anti-MEK1 antibody |
| 中文名称 | MEK1重组兔单抗 |
| 别 名 | mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK1; Map2k1; MAPKK1; Mek1; MEKK1; Prkmk1; MP2K1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:20-100,IHC-F=1:20-100,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MEK1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | PBS, Glycerol, BSA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. SWISS: Q02750 Gene ID: 5604 Database links: Entrez Gene: 5604 Human Entrez Gene: 26395 Mouse Omim: 176872 Human SwissProt: Q02750 Human SwissProt: P31938 Mouse Unigene: 145442 Human Unigene: 248907 Mouse Unigene: 5850 Rat
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| 产品图片 |
Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Muscle tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human MDA-MB-231 cell lysates
Lane 7: Human A431 cell lysates
Lane 8: Human Jurkat cell lysates
Primary: Anti-MEK1 (bsm-60744R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
