| 产品编号 | bsm-34123M |
| 英文名称 | Cytokeratin 15 |
| 中文名称 | 细胞角蛋白15单克隆抗体 |
| 别 名 | K1C15_HUMAN; Cytokeratin-15; CK 15; CK-15; CK15; K15; K1CO; Keratin 15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; Keratin15; KRT 15; KRTB; KRTL15; keratin-15, basic; Type I cytoskeletal 15. |
| 研究领域 | 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 49kDa |
| 细胞定位 | 细胞核 细胞浆 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytokeratin 15 |
| 亚 型 | IgG1, Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq]. SWISS: P19012 Gene ID: 3866 Database links: Entrez Gene: 3866 Human Entrez Gene: 16665 Mouse Omim: 148030 Human SwissProt: P19012 Human SwissProt: Q61414 Mouse Unigene: 654570 Human Unigene: 440010 Mouse Unigene: 153568 Rat
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| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (bsm-34123M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (bsm-34123M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (bsm-34123M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
