| 产品编号 | bsm-60707R |
| 英文名称 | APEX1 Recombinant Rabbit mAb |
| 中文名称 | 多功能DNA修复酶重组兔单抗 |
| 别 名 | APEX1_HUMAN; DNA-(apurinic or apyrimidinic site) endonuclease; EC:3.1.11.2; APE; APE1; APEX; APX; HAP1; REF1; APEX nuclease(APEN); Apurinic-apyrimidinic endonuclease 1(AP endonuclease 1; APE-1); REF-1; Redox factor-1; DNA-(apurinic or apyrimidinic site) e |
| 研究领域 | 心血管 染色质和核信号 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | R2D12 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:200-500,IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APEX1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.[provided by RefSeq, Dec 2019] Subcellular Location: Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Note=Detected in the cytoplasm of B-cells stimulated to switch. Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. DNA-(apurinic or apyrimidinic site) lyase, mitochondrial: Mitochondrion. Note=The cleaved APEX2 is only detected in mitochondria. Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress. SWISS: P27695 Gene ID: 328 Database links:
Entrez Gene: 328 Human Entrez Gene: 11792 Mouse SwissProt: P27695 Human SwissProt: P28352 Mouse |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HEK-293, 2: HepG2, 3: NIH/3T3, 4:
RAW264.7
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 35 kDa
Observed MW: 37 kDa
Tissue: Rat liver
Section type: Formalin-fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-60707R
Tissue: Mouse liver
Section type: Formalin-fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-60707R
Tissue: Human ovarian carcinoma
Section type: Formalin-fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for
bsm-60707R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
