| 产品编号 | bsm-60663R |
| 英文名称 | Rabbit Anti-Histone H2A (Acetyl-Lys9) antibody |
| 中文名称 | 乙酰化组蛋白H2A兔单克隆抗体 |
| 别 名 | Acetyl-Histone H2A (Lys9); H2AK9ac; H2a 615; H2A; H2A GL101; H2A histone family member A; H2A.1; H2A.2; H2A/a; H2A/m; H2A/O; H2A/q; H2AFA; H2AFE; H2AFL; H2AFM; H2AFO; H2AFQ; HIST1H2AE; HIST1H2AJ antibody HIST2H2AA; HIST2H2AA3; HIST2H2AB; HIST2H2AC; Histone 1 H2ae; Histone 2 H2aa3; Histone 2 H2ab; Histone 2 H2ac; Histone H2A type 1 B; Histone H2A type 1 C; Histone H2A type 1 E; Histone H2A type 1 J; MGC74460. |
| 产品类型 | 乙酰化抗体 重组兔单抗 |
| 研究领域 | 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | R5F7 |
| 交叉反应 | (predicted: Human,Mouse) |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 细胞浆 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H2A (Acetyl-Lys9) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. SWISS: Q16777 Gene ID: 3012 Database links:
Entrez Gene: 3012 Human Entrez Gene: 317772 Human Entrez Gene: 8335 Human Entrez Gene: 8337 Human Entrez Gene: 8338 Human Entrez Gene: 319166 Mouse Omim: 142720 Human Omim: 602786 Human Omim: 602797 Human SwissProt: P04908 Human SwissProt: P28001 Human SwissProt: P35065 Human SwissProt: Q93077 Human SwissProt: Q99878 Human SwissProt: P10812 Mouse SwissProt: P22752 Mouse Unigene: 121017 Human Unigene: 248174 Human Unigene: 408067 Human Unigene: 417332 Human Unigene: 434195 Human Unigene: 261665 Mouse
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| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: (-): NIH/3T3, (+): NIH/3T3+ trichostain
A (1μM, 18 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 KDa
Observed MW: 14 KDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium
butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 KDa
Observed MW: 14 KDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
