产品编号 | bsm-60651R |
英文名称 | Rabbit Anti-BLBP antibody |
中文名称 | 脑型脂肪酸结合蛋白重组兔单抗 |
别 名 | Fatty acid-binding protein brain; FABP; BFABP; Brain lipid binding protein; DKFZp547J2313; FABP 7; BLBP; FABPB; Fatty Acid Binding Protein 7; Fatty acid binding protein 7 brain; Fatty acid binding protein, brain; Mammary derived growth inhibitor related; MRG; BP7_HUMAN. |
研究领域 | 信号转导 干细胞 转录调节因子 G蛋白偶联受体 |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | R2D5 |
交叉反应 | Human,Mouse |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:50-1:100,IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 15 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BLBP |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in brain and other neural tissues. SWISS: O15540 Gene ID: 2173 Database links: Entrez Gene: 2173 Human Entrez Gene: 12140 Mouse SwissProt: O15540 Human SwissProt: P51880 Mouse |
产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Mouse spleen(-), 2: Mouse brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 13 kDa
Tissue: Mouse cerebrum
Section type: Formalin-fixed & Paraffin
-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60651R
Tissue: Human cerebrum
Section type: Formalin-fixed & Paraffin
-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60651R
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |