| 产品编号 | bs-41306R |
| 英文名称 | NPHS2 Rabbit pAb |
| 中文名称 | 肾小球裂孔膜蛋白PDCN抗体 |
| 别 名 | PDCN; SRN1; podocin; PODO_HUMAN; NPHS2; PODO_MOUSE; PODO_RAT; |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Podocin protein: 125-383/383 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. SWISS: Q9NP85 Gene ID: 7827 Database links: Entrez Gene: 7827 Human Entrez Gene: 170484 Mouse Omim: 604766 Human SwissProt: Q9NP85 Human SwissProt: Q91X05 Mouse Unigene: 412710 Human Unigene: 289099 Mouse Unigene: 86433 Rat |
| 产品图片 |
Sample:
Lane 1: Recombinant human Podocin, N-his
Primary: Anti-NPHS2 (bs-41306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 30 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag (Cat. bs-41306P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 155.5 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human NPHS2 protein, His Tag (Cat. bs-41306P) at 2 μg/mL (100 μL/well) can bind Rabbit Anti-Human NPHS2 Antibody, the EC50 is 374.9 ng/mL.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
