| 产品编号 | bsm-54036R |
| 英文名称 | Rabbit Anti-Periostin antibody |
| 中文名称 | 成骨细胞特异性因子2重组兔单抗 |
| 别 名 | OSF 2; OSF2; osteoblast specific factor 2 (fasciclin I-like); Osteoblast specific factor 2; Osteoblast specific factor; PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2; Periostin isoform thy4; Periostin isoform thy6; Periostin isoform thy8; Periostin osteoblast specific factor; PN; POSTN; POSTN_HUMAN. |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 转录调节因子 细胞粘附分子 血管内皮细胞 肿瘤细胞生物标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1:20-50,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 100kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Periostin |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Periostin is a disulfide linked 90 kDa, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta 1(TGF beta 1) and bone morphogenetic protein (BMP2). Periostin has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart. Abnormal expression of periostin is also linked to angiogenesis and metastatsis in epithelial tumors. SWISS: Q15063 Gene ID: 10631 Database links: Entrez Gene: 10631 Human Entrez Gene: 50706 Mouse Omim: 608777 Human SwissProt: Q15063 Human SwissProt: Q62009 Mouse Unigene: 136348 Human Unigene: 721018 Human Unigene: 236067 Mouse Unigene: 30516 Rat |
| 产品图片 |
Western blot analysis of Periostin on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (bsm-54036R, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 93 kDa
Observed band size: 80 kDa
Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Periostin Monoclonal Antibody, Unconjugated(bsm-54036R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Flow cytometric analysis of Periostin was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54036R, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
