| 产品编号 | bsm-54264R |
| 英文名称 | Rabbit Anti-SET antibody |
| 中文名称 | SET易位蛋白/髓系白血病相关蛋白重组兔单抗 |
| 别 名 | SET_HUMAN; Protein SET; HLA-DR-associated protein II; Inhibitor of granzyme A-activated DNase (IGAAD); PHAPII; Phosphatase 2A inhibitor I2PP2A (I-2PP2A); Template-activating factor I (TAF-I); 2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA; |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 细胞凋亡 细胞周期蛋白 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,ICC/IF=1:50-200,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 32kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SET protein : C-terminal |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. SWISS: Q01105 Gene ID: 6418 Database links: Entrez Gene: 6418 Human Entrez Gene: 56086 Mouse Entrez Gene: 671392 Mouse SwissProt: Q01105 Human SwissProt: Q9EQU5 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
