| 产品编号 | bsm-60218R |
| 英文名称 | Keratin 7 |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | B10D5 |
| 交叉反应 | (predicted: Human) |
| 产品应用 | WB=1:500-1:2000, IHC-P=1:100-500, ICC=1:50-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 角蛋白（细胞角蛋白）是主要在上皮细胞中表达的中间丝蛋白。角蛋白的异二聚体由一个酸性角蛋白（或 I 型角蛋白，角蛋白9 至 23）和碱性角蛋白（或 II 型角蛋白，角蛋白 1 至 8）组成聚合形成纤丝。角蛋白同工型的组织特异性和分化特异性表达，使其可用作研究生物标记物。研究证实，角蛋白基因中的突变与皮肤疾病、肝与胰疾病和炎性肠病相关。角蛋白 7 和角蛋白 19 存在于肝脏和胰腺祖细胞/干细胞中。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Lysate: Hela Protein loading quantity: 20 μg Exposure time: 1 s Predicted MW: 51 kDa Observed MW: 51 kDa 
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60218R 
Tissue: Human breast cancer
Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary incubation condition: 1 hour at room temperature Counter stain: Hematoxylin Comment: Color brown is the positive signal for bsm-60218R |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
