| 产品编号 | bs-60168R |
| 英文名称 | Butyryl-Histone H2B (Lys5) Rabbit pAb |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-1:2000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Antigen affinity purification |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白经过各种酶催化的修饰，包括乙酰化，甲基化，磷酸化，泛素化等。赖氨酸丁酰化是新发现的一种控制染色体结构和基因转录的可逆修饰。赖氨酸丁酰化在从蠕虫到人的真核细胞中高度保守。组蛋白赖氨酸丁酰化独特的结构和基因组定位表明，它在机制和功能上不同于组蛋白赖氨酸乙酰化。具体地说，在人类体细胞和兔雄性生殖细胞基因组中，组蛋白丁酰化标志着活性启动子或潜在增强子。组蛋白H3在Lys18位点的丁酰化可能在表观遗传调控中发挥重要作用，包括染色质重塑和DNA转录调控。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
Cell line: (A) HeLa, (B) HeLa+SBA (5mM, 24hr)
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bs-60168R
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
