| 产品编号 | bs-60081R |
| 英文名称 | Rabbit Anti-Histone H3 (tri methyl K4, serotonyl Q5) antibody |
| 中文名称 | |
| 产品类型 | 甲基化抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | Antigen affinity purification |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 组蛋白翻译后修饰（PTM）是表观遗传学的关键机制，可调节染色质结构，称为“组蛋白密码”。血清素也称为5-羟色胺（5-HT），是一种兴奋性神经递质，可激活大脑中特定的膜受体来调节脑细胞的活动。在TGM2蛋白的催化下，5-羟色胺的氨基可与组蛋白H3第5位谷氨酰胺的侧链羧基共价结合，形成组蛋白5-羟色胺（H3Q5ser）。H3Q5ser与相邻的H3K4me3间相互影响。H3K4me3Q5ser可增强TFIID等转录因子与染色质的结合，从而激活发育中的啮齿动物大脑或人类神经元中下游基因的表达。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: lane 1: PTM-1420+H3K4me3/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: N2a
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
