| 产品编号 | bsm-60242M |
| 英文名称 | Mouse Anti-COX1/MTCO1 antibody |
| 中文名称 | 细胞色素c氧化酶1单克隆抗体 |
| 别 名 | COX1_HUMAN; Cytochrome c oxidase subunit 1; EC:7.1.1.9; Cytochrome c oxidase polypeptide I; MT-CO1; COI; COXI; COX I; MTCO1; |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | E7A11 |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Contributes to cytochrome-c oxidase activity. Predicted to be involved in electron transport coupled proton transport and mitochondrial electron transport, cytochrome c to oxygen. Part of mitochondrial respiratory chain complex III and mitochondrial respiratory chain complex IV. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. SWISS: P00395 Gene ID: 4512 Database links: Entrez Gene: 4512 Human Entrez Gene: 17708 Mouse SwissProt: P00395 Human SwissProt: P00397 Mouse 细胞色素c氧化酶的组成部分，是线粒体电子传递链中最后一个推动氧化磷酸化的酶。呼吸链包含3个多亚单位复合物琥珀酸脱氢酶（复合体II，CII）、泛醌细胞色素c氧化还原酶（细胞色素b-c1复合物，复合体III，CIII）和细胞色素c氧化酶（复合体IV，CIV），它们协同将NADH和琥珀酸衍生的电子转移到分子氧中，在内膜上形成一个电化学梯度来驱动跨膜转运和ATP合成酶。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Lysate: HeLa, MCF-7, N2a, BRL
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 57 kDa
Observed MW: 35 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
