| 产品编号 | bsm-60618R |
| 英文名称 | SATB1 Recombinant Rabbit mAb |
| 中文名称 | |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | H6D4 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-1:2000,IHC-P=1:100-500,Flow-Cyt=1:20-1:100,ICC/IF=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 促成 Xist RNA 介导的 X 失活启动的关键沉默因子，发生在胚胎发生和淋巴瘤中。在富含 AT 的特殊序列（共有 SATB1 结合序列 (CSBS)，在核基质或支架相关区域）与 DNA 结合。认为识别双链 DNA 的糖磷酸结构。通过与 DNA 的基质附着区 (MAR) 结合并诱导局部染色质环重塑，以磷酸化和乙酰化状态依赖性方式控制核和病毒基因表达的转录抑制因子。作为几种染色质重塑酶（例如 MHC-I 位点的 PML）的停靠位点，还可以直接向启动子和增强子募集辅抑制因子 (HDAC) 或辅激活因子 (HAT)。调节对来自胸腺细胞的免疫 T 细胞 CD8SP 的成熟至关重要的基因。需要转换胎儿珠蛋白种类，以及在红细胞分化过程中调节 β 和 γ 珠蛋白基因。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Jurkat, 2: THP-1
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 86 kDa
Observed MW: 100 kDa
Tissue: Human tonsil
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for bsm-60618R
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60618R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
