| 产品编号 | bsm-60469M |
| 英文名称 | Mouse Anti-STRAP antibody |
| 中文名称 | 丝氨酸/苏氨酸激酶受体相关蛋白单克隆抗体 |
| 别 名 | MAP activator with WD repeats; MAWD; PTWD; serine/threonine kinase receptor associated protein; STRAP; UNR-interacting protein; UNRIP; WD 40 repeat protein PT WD; STRAP_HUMAN. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 转录调节因子 激酶和磷酸酶 |
| 抗体来源 | Mouse |
| 克隆类型 | Recombinant |
| 克 隆 号 | F5B7 |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,ICC/IF=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STRAP |
| 亚 型 | IgG1/Kappa |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium; negative regulation of transforming growth factor beta receptor signaling pathway; and spliceosomal snRNP assembly. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm. [provided by Alliance of Genome Resources, Apr 2022] SWISS: Q9Y3F4 Gene ID: 11171 SMN复合物在小核核糖核蛋白（snRNPs）的组装中起催化作用，snRNPs是剪接体的组成部分。因此，在细胞前mRNAs的拼接中起着重要的作用。大多数剪接体snRNP包含一组共同的Sm蛋白质SNRPB、SNRPD1、SNRPD2、SNRPD3、SNRPE、SNRPF和SNRPG，它们在小核RNA的Sm位点上组装成七聚体蛋白环，形成核心snRNP。在细胞质中，Sm蛋白SNRPD1、SNRPD2、SNRPE、SNRPF和SNRPG被控制核心snRNP组装的伴侣CLNS1A捕获在非活性的6S pICln Sm复合物中。CLNS1A的SMN复合物从捕获的Sm蛋白中解离并将其转移到SMN-Sm复合物触发核心snRNPs的组装并将其转运到细胞核。条带在SMN复合体的细胞分布中起作用。负调节TGF-β信号，但通过增强其自身磷酸化和显著减少PDPK1与14-3-3蛋白的结合来正调节PDPK1激酶活性。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 38 kDa
Observed MW: 38 kDa
Tissue: Human-lung-squamous-carcinoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60469M
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60469M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60469M
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
