| 产品编号 | bsm-60525R |
| 英文名称 | PRMT5 Recombinant Rabbit mAb |
| 中文名称 | 组蛋白精氨酸甲基转移酶5重组兔单抗 |
| 别 名 | ANM5_HUMAN; Protein arginine N-methyltransferase 5; HRMT1L5; IBP72; JBP1; SKB1; EC:2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog(SKB1 homolog; SKB1Hs); Prot |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | D1B12 |
| 交叉反应 | Human |
| 产品应用 | WB=1:2000-1:10000,IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 70 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG/Kappa |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] 精氨酸甲基转移酶，既能催化omega-N单甲基精氨酸（MMA）和对称二甲基精氨酸（sDMA）的形成，又能优先催化MMA的形成。特异性介导小核核糖核蛋白smd1（SNRPD1）和smd3（SNRPD3）中精氨酸残基的对称二甲基化；这种甲基化是snRNP核心粒子组装和生物生成所必需的。甲基化SUPT5H并可能调节其转录延伸特性。髓鞘碱性蛋白（MBP）精氨酸残基的单酸和二甲基化。可能在细胞因子激活的转导途径中发挥作用。负调控细胞周期素E1启动子活性和细胞增殖。在生殖细胞发育过程中甲基化组蛋白H2A和H4'Arg-3'。甲基化组蛋白H3'Arg-8'，可能抑制转录。甲基化Piwi蛋白（PIWIL1，PIWIL2和PIWIL4），Piwi蛋白甲基化是与包含Tudor结构域的蛋白质相互作用和随后定位到减数分裂的细微差别所必需的。 |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: HepG2
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 73 kDa
Observed MW: 70 kDa
Tissue: Human breast cancer
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60525R
Tissue: Human liver
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60525R
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
