| 产品编号 | bsm-60237R |
| 英文名称 | CD43 Recombinant Rabbit mAb |
| 中文名称 | CD43重组兔单抗 |
| 别 名 | LEUK_HUMAN; Leukosialin; SPN; GPL115; GALGP; GPL 115; Galactoglycoprotein(GALGP); Leukocyte sialoglycoprotein; Sialophorin; CD43 cytoplasmic tail; CD 43; CD43 antigen; |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | H6G11 |
| 交叉反应 | Human |
| 产品应用 | WB=1:10000-1:20000,Flow-Cyt=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 38 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 0.5mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017] Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. Database links: Entrez Gene: 6693 Human Entrez Gene: 20737 Mouse SwissProt: P16150 Human SwissProt: P15702 Mouse CD43又名白细胞唾液酸糖蛋白或唾液酸蛋白，是一种跨膜表面蛋白，在人体中有SPN（唾液蛋白）基因编码。CD43分子中的缺陷与Wiskott-Aldrich综合征的发病有关。 |
| 产品图片 |
Sample:
Lane 1: Human U937 cell lysates
Lane 2: Human K562 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-CD43 (bsm-60237R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 105 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
