产品编号 | bsm-60426R |
英文名称 | QSOX1 Recombinant Rabbit mAb |
中文名称 | 巯基氧化酶1抗体 |
别 名 | QSOX1_HUMAN; Sulfhydryl oxidase 1; QSCN6; EC:1.8.3.2; hQSOX; Quiescin Q6; UNQ2520/PRO6013; quiescin sulfhydryl oxidase 1; Q6; |
抗体来源 | Rabbit |
克隆类型 | Recombinant |
克 隆 号 | H12B2 |
交叉反应 | Human |
产品应用 | WB=1:500-1:1000,Flow-Cyt=1:50-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 80 kDa |
检测分子量 | |
细胞定位 | 细胞外基质 |
性 状 | Liquid |
浓 度 | 1mg/ml |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 | QSOX1属于FAD依赖的巯基氧化酶家族,是一种非典型的二硫化物催化剂。QSOX1主要定位于人类胚胎成纤维细胞中的高尔基体和内质网(ER),它与蛋白质二硫键异构酶(PDI)协同作用,以帮助将新生蛋白质折叠在细胞中。QSOX1主要在心脏,胎盘,肺,肝,骨骼肌,胰腺中表达,在脑和肾脏中表达非常弱。有研究发现,肿瘤细胞中QSOX1表达的增加,使肿瘤细胞能够积极规避由活性氧介导的细胞凋亡机制。 |
产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: MCF-7, 2: MDA-MB-231,3: T47D, 4: 293T
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 83 kDa
Observed MW: 67 kDa
Cell line: MDA-MB-231
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60426R
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |