| 产品编号 | bsm-60613R |
| 英文名称 | CD25 Recombinant Rabbit mAb |
| 中文名称 | 白介素2受体a链/ICD25重组兔单抗 |
| 别 名 | Interleukin 2 receptor alpha chain; CD 25; CD25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL2 RA; IL-2RA; IL2R; IL2RA; IL2RAC; Interleukin 2 Receptor alpha; Interleukin 2 receptor alpha chain; Interleukin 2 receptor; Ly43; p55; T Cell G |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | H12C8 |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | 白细胞介素 2 (IL2) 受体α (IL2RA) 和β (IL2RB) 链与共同的γ 链(IL2RG) 一起构成高亲和力IL2 受体。同源二聚体 α 链 (IL2RA) 产生低亲和力受体，而同源二聚体 β (IL2RB) 链产生中等亲和力受体。通常，一种完整的膜蛋白、可溶性 IL2RA 已被分离并确定是由细胞外蛋白水解产生的。交替剪接的 IL2RA mRNA 已被分离出来，但每个的意义目前尚不清楚。该基因的突变与白细胞介素 2 受体 α 缺陷有关。2019 年严重冠状病毒病 (COVID-19)（由新型严重急性呼吸系统综合症冠状病毒 2（SARS-CoV-2）引起的疾病）患者血浆中的 IL2R 水平显着升高。同样，发现不同类型癌症患者的血清 IL-2R 水平升高。某些 IL2RA 和 IL2RB 基因多态性与肺癌风险相关。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human lymphoid); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD25) Monoclonal Antibody, Unconjugated (bsm-60613R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD25) Monoclonal Antibody, Unconjugated (bsm-60613R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
