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Fibrillarin Recombinant Rabbit mAb (bsm-60560R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-60560R
英文名称 Fibrillarin Recombinant Rabbit mAb
中文名称
别    名 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL_HUMAN; FIB; FIB1; FLRN; Nop1p; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2''-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin.  
抗体来源 Rabbit
克隆类型 Recombinant
克 隆 号 H4C3
交叉反应 Human,Mouse,Rat
产品应用 WB=1:500-2000,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 kDa
检测分子量
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
亚    型 IgG/Kappa
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 S-腺苷-L-甲硫氨酸依赖的甲基转移酶,具有使RNA和蛋白质甲基化的能力。通过催化前核糖体RNA中核糖部分的位点特异性2'-羟基甲基化参与前rRNA加工。位点特异性由与底物碱基配对的引导RNA提供。甲基化发生在与引导RNA碱基配对的序列的特征距离处。可能催化C/D-RNP复合物中U6-snRNAs的2'-O-甲基化。U6-snRNA 2'-O-甲基化是mRNA剪接保真度所必需的。还通过介导组蛋白H2A(H2AQ104me)的“Gln-105”甲基化而充当蛋白质甲基转移酶,这是一种损害FACT复合物结合的修饰,并特异性存在于35S核糖体DNA位点。
产品图片
25 ug total protein per lane of various lysates (see on figure) probed with Fibrillarin monoclonal antibody, unconjugated (bsm-60560R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FBL) monoclonal Antibody, unconjugated (bsm-60560R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-FBL antibody (bsm-60560R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488(bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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